Goals of workshop

• Job Boekhoven
• Kerstin Göpfrich
• Christoph Weber

Creating links between physics and chemistry to reconstruct the Origins of Life

• Dieter Braun

Introduction: The idea of group discussions and perspective writing

Group discussion: What is the goal of engineering life? collaborator meetings, jointly writing a perspective article on “Challenges on Engineering Life”

Selected short talk - "Coacervate protocells formed by native chemical ligation"

• Annemiek Slootbeek

Selected short talk - "Cycles of (de)hydration far away from equilibrium catalyses chemical processes"

• Ivar Haugerud

Selected short talk - "Chirality transfer from a 3D macro shape to the molecular level through asymmetric secondary flows"

• Alessandro Sorrenti

From self-organization to self-division

• Petra Schwille

Group Discussion: Life is functional evolution. But how to define & measure function?

Selected short talk - "Ghost in the cell: artificial cells via enzyme-mediated polymer synthesis and self-assembly"

• Andrea Belluati

Selected short talk - "Linking molecular-scale to mesoscale dynamics in biomolecular condensates"

• Nicola Galvanetto

Selected short talk - "Toward building an artificial nucleus"

• Eva Bertosin

Collective intelligence - from bugs to bots

• L Mahadevan

Group Discussion: Engineered biotectonics - from cells to ecosystems

Molecular information processing in chemical reaction networks

• Wilhelm Huck

Group Discussion

Selected short talk - "Systems chemistry of aminoacyl phosphates: Spontaneous and selective peptide oligomerisation in water driven by phase changes."

• Kun Dai

Selected short talk - "Heat flows purify >50 building blocks of life and boost their reactivity"

• Thomas Matreux

Selected short talk - "Reciprocal Coupling in Chemically Fueled Assembly: A ReactionCycle Regulates Self-Assembly and Vice Versa"

• Brigitte Kriebisch

General Group discussion: Should synthetic cells have synthetic hardware?

General Group discussion: Joint perspective writing

Physics of Active Emulsions

• Frank Jülicher

Group Discussion: How to use non-equilibrium physics to create life-like behaviors?

Selected short talk - "Ordered structures from liquid-liquid phase separation by supramolecular polymerizations"

• Hailin Fu

Selected short talk - "Lesion-Induced DNA Amplification as a Model System for Autonomous Nucleotide Self-Replication"

• Juli Gibbs

Selected short talk - "A DNA segregation module for synthetic cells"

• Mai Tran

An RNA world of possibilities: the satellite, the robot and the replicase

• Ebbe Sloth Andersen

Group Discussion: “How do we design RNA systems that sense, compute, and act? And how do we make them replicate, adapt, and evolve.”

Hike with short talks and lunch at mountain hut

Bridging the RNA and lipid worlds: unexpected roles for lipids in RNA regulation

• James Saenz

Group discussion: How do we program heritable organization into synthetic life?

Selected short talk - "Key features in the design and fabrication of living robots"

• Maria Guix

Information processing in simple organisms: A case study

• Karen Alim

Selected short talk - "Quasi-Laminar Active Nematic Flows Under Confinement"

• Olga Bantysh

Communicating Active Matter

• Erwin Frey

Group discussion: Can theory contribute to the field of soft robotics, and what is soft robotics anyway?

Cell-free protein synthesis: the engine of synthetic cells?

• Nadanai Lauhakunakorn

Group Discussion: How should we build and sustain self-regenerating systems?

Selected short talk - "Phase-separated compartments as biochemical reactors"

• Sudarshana Laha

Selected short talk - "DNA origami and DNA droplet components for synthetic cells"

• Shelley Wickham

Selected short talk - "Influence of the extracellular domain size on the dynamic behavior of membrane proteins"

• Cenk Gurdap

General Group discussion: Open questions on perspective writing

How dynamic molecular networks can accumulate life-like properties

• Sijbren Otto

Group discussion: How to tame chemistry?

Selected short talk - "Template-based information transfer in chemically fueled dynamic combinatorial libraries"

• Christine Kriebisch

Selected short talk - "Reaction-Directed Assembly: Controlling Changes of Membrane Topology by Reaction Cycles"

• Gregor Ibbeken

Selected short talk -“TBD”

• Alexander Bergmann

Spatial proximity effects for engineering life

• Ulrich Gerland

Group discussion: How can we exploit spatial arrangement for engineering life?

A lesson from early cells

• Claudia Bonfio

Group Discussion: Compartmentalisation: natural vs. artificial building blocks

Selected short talk - "Exploring the role of class I myosins in plasma membrane organization using an in vitro reconstitution approach"

• Bhagyashri Mahajan

Selected short talk - "The dynamics of phase separation and wetting in cell-free expression system"

• Shuzo Kato

Selected short talk - "Bottom-up building a self-regenerating microfluidic biochemical constructor"

• Pao-Wan Lee

Life but not alive: bioengineering with synthetic cells

• Kate Adamala

Group discussion: How can we build an interoperable synthetic cell systems?

Closing remarks

• Christoph Weber
• Job Boekhoven
• Kerstin Göpfrich